Recombinant H5N1 NA, His-tagged NA-190O

Recombinant H5N1 NA, His-tagged

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Recombinant H5N1 NA, His-tagged

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Cat.No. : NA-190O
Product Overview : Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) (Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA)) His 36 - Lys 449 (Accession # H8PF47) was produced in human 293 cells (HEK293).
Description : Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
Source : HEK293
Species : H5N1
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity : Measured by its ability to cleave a fluorogenic substrate, 2"-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2"-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at
Molecular Mass : Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. DTT-reduced Protein migrates as 48 kDa in SDS-PAGE.
Endotoxin : Less than 1.0 EU per μg of the Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Reconstitution : See Certificate of Analysis for reconstitution instructions and specific concentrations.
Gene Name : NA neuraminidase [ Influenza A virus (A/goose/Guangdong/1/1996(H5N1)) ]
Official Symbol : NA
Synonyms : NA, Neuraminidase, Influenza A, H5N1
Gene ID : 3654619
mRNA Refseq : NC_007361.1
Protein Refseq : YP_308668.1

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