Recombinant human biotinylated MAP2K4, His-tagged

• Cat.No.: MAP2K4-33H
• Product Overview: Recombinant human biotinylated MAP2K4, N-terminally fused to HIS6, was expressed in E.coli. Biotinylated protein is not phosphorylated and activated by MKKs. It is suitable as substrate for kinase activity assays and for western blot analyses.

Specification

• Description: MKK4 is a dual specificity kinase belonging to the group of MAP kinase kinase (MKKs). MKKs are one part of three-kinase phosphorelay system "MAPK cascade" that converts extra cellular stimuli into various cellular responses such as gene expression, mitosis, and differentiation, proliferation, and cell survival/apoptosis. MKK4 as well as MKK7 are critical upstream activators of JNK signalling required for developmental programmes and responses to various extracellular stimuli. Upon phosphorylation at Ser and Thr residues within a Ser-X-Ala-Lys-Thr motif in their activation loops by MKKKs (e.g. ASK, MEKK, MLK, and TAK) activated MKK4 is able to in turn activate JNK by dual phosphorylation of the Thr-Pro-Tyr motif. Biochemical analyses of JNK signalling revealed, that MKK4 and MKK7 cooperate in activation process of JNK. Upon phosphorylation of the Tyr residue mediated by MKK4 full activation of JNK is achieved by Thr-phosphorylation triggered by MKK7. Whereas MKK3 and MKK6 are specific p38MAPK activators different studies showed that p38α is regulated by MKK4 too. MKK4 is involved in a variety of physiological and pathophysiological processes, especially in the nervous system, heart, immune system and the development of cancer.Recent studies revealed, that over expression of the mkk4 gene in an ovarian cancer line is linked with a reduced proliferative activity and increased apoptosis, indicating that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 20 % glycerol, pH 8.0.
• Molecular Mass: 45.9 kDa
• Purity: >90% (The purified fusion protein was analyzed on Coomassie stained SDS gel.)
• Storage: - 80°C (avoid repeated freeze-thaw cycles !)
• Concentration: 0.19 mg/ml

Gene Information

• Gene Name: MAP2K4 mitogen-activated protein kinase kinase 4 [ Homo sapiens ]
• Official Symbol: MAP2K4
• Synonyms: MAP2K4; mitogen-activated protein kinase kinase 4; SERK1; dual specificity mitogen-activated protein kinase kinase 4; JNKK1; MEK4; MKK4; PRKMK4; MEK 4; MAPKK 4; SAPK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK activating kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; stress-activated protein kinase kinase 1; JNKK; SEK1; MAPKK4; SAPKK1; SAPKK-1
• Gene ID: 6416
• mRNA Refseq: NM_003010
• Protein Refseq: NP_003001
• MIM: 601335
• UniProt ID: P45985
• Chromosome Location: 17p12
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Ceramide signaling pathway, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity