Recombinant Human EFNA3 protein, His-tagged
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|Product Overview :||Recombinant Human EFNA3 (Gln23-Ser213) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Ephrin-A3 (Ephrin A3) is also known as EFL-2, EHK1 ligand, EHK1-L, EPH-related receptor tyrosine kinase ligand 3, EFL2, EPLG3 and LERK3,which comprises the largest subfamily of receptor protein-tyrosine kinases (RTKs), and has been involved in a variety of biological processes, especially in the nervous system and in erythropoiesis, such as axon guidance and topographic map formation, synaptic plasticity, angiogenesis, and meanwhile have possible contributions to tumor growth and metastasis. Ephrin A3 is cell surface GPI-bound ligand for Eph receptors and belongs to the family of receptor tyrosine kinases. Ephrin can bind promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells.|
|Predicted N Terminal :||Gln23|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 22.2 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Gln23-Ser213|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of EFNA3-155H :||
Human Ephrin-A3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Gene Name :||EFNA3|
|Official Symbol :||EFNA3|
|Synonyms :||EFNA3; ephrin-A3; EPLG3; Ehk1 L; LERK3; EFL-2; LERK-3; EHK1 ligand; ligand of eph-related kinase 3; eph-related receptor tyrosine kinase ligand 3; EFL2; Ehk1-L|
|Gene ID :||1944|
|mRNA Refseq :||NM_004952|
|Protein Refseq :||NP_004943|
|UniProt ID :||P52797|
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