Active Recombinant Human CUL1/RBX1
Cat.No. : | CUL1-146H |
Product Overview : | Recombinant human CUL1 (amino acid residues 1-776), RBX1 (amino acid residues 1-115) was expressed in Insect (Sf21) cells. |
- Specification
- Gene Information
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Description : | Cullin-RING-Ligases (CRLs) are one largest class of ubiquitin E3 ligases and the enzymes of the NEDDylation pathway play a pivotal role in the activation of these, akin to ubiquitylation, the E1 activating enzyme (APP-BP1/UBA3 heterodimer) and the E2 conjugating enzymes (UBE2M or UBE2F) are involved in mammalian NEDDylation of the Cullin Ring Ligases (CRLs) (Meyer-Schaller et al., 2009; Huang et al., 2011; Morimoto et al., 2003). |
Source : | Insect cells |
Species : | Human |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol. |
Bio-activity : | E3 Ligase Assay: The activity of Cul1/Rbx1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL2 and thioester-loaded His-Ube2M-NEDD8. Incubation of Cul1/Rbx1 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of His-DCNL2 at 4?C was compared at two time points T0 and T2 minutes. Neddylation of the Cul1 subunit in the presence of His-DCNL2 was demonstrated. |
Molecular Mass : | Cul1: ~89.9 kDa; Rbx1: ~12.3 kDa |
Purity : | >95% by InstantBlue™ SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 0.5 mg/ml |
Gene Name : | CUL1 cullin 1 [ Homo sapiens ] |
Official Symbol : | CUL1 |
Synonyms : | CUL1; cullin 1; cullin-1; CUL-1; MGC149834; MGC149835; |
Gene ID : | 8454 |
mRNA Refseq : | NM_003592 |
Protein Refseq : | NP_003583 |
MIM : | 603134 |
UniProt ID : | Q13616 |
Chromosome Location : | 7q36.1 |
Pathway : | APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Cell Cycle, organism-specific biosystem; |
Function : | protein binding; ubiquitin protein ligase binding; |
Products Types
◆ Recombinant Protein | ||
CUL1-688H | Recombinant Human CUL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
CUL1-2080M | Recombinant Mouse CUL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Cul1-2374M | Recombinant Mouse Cul1 Protein, Myc/DDK-tagged | +Inquiry |
CUL1-648H | Recombinant Human CUL1 Protein, His-tagged | +Inquiry |
CUL1-2239H | Recombinant Human CUL1 Protein (Met1-Glu250), His tagged | +Inquiry |
◆ Lysates | ||
CUL1-7185HCL | Recombinant Human CUL1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionTechniques such as immunohistochemistry, Western blotting, and gene expression analysis are commonly used to study CUL1 protein expression and function in clinical research.
Yes, CUL1 protein has been implicated in various diseases, including neurodegenerative disorders and cardiovascular diseases, due to its role in protein degradation and cellular regulation.
While CUL1 protein levels are altered in certain cancers, it is not commonly used as a standalone biomarker. However, it may be part of a panel of markers used for cancer diagnosis.
Yes, mutations in the CUL1 gene have been linked to a rare genetic disorder called Cullin-Ring Ligase 1 Syndrome, which is characterized by developmental delays and intellectual disabilities.
Dysregulation of CUL1 protein can lead to the abnormal degradation of cell cycle regulators, resulting in uncontrolled cell division and potentially contributing to tumorigenesis.
Customer Reviews (3)
Write a reviewI highly recommend the CUL1 protein for a variety of research applications.
It demonstrates excellent performance in ELISA, consistently delivering accurate and reliable results.
the CUL1 protein has proven to be an invaluable tool in protein electron microscopy structure analysis.
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