| Cat. No. : |
GLO1-125H |
| Description : |
Glyoxalase I, also known as GLO1, belongs to the glyoxalaseⅠfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. |
| Sequences of amino acids : |
MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM |
| Purity : |
> 90% by SDS - PAGE |
| Form : |
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol |
| Molecular Weight : |
20.7 kDa (184 aa), confirmed by MALDI-TOF |
| Concentration : |
1 mg/ml (determined by Bradford assay) |
| Biological activity : |
Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25C. Specific activity was expressed in units/mg of protein. |
| Activity Assay : |
1. Prepare a 1.5ml reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin. 2. Equilibrate to 25C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer. 3. Add 50ul of recombinant glyoxalase I solution with various concentrations (0.5ug, 1ug, 2ug) in 1.4ml reaction buffer. 4. Immediately mix by inversion and record the increase at A240nm for 5 minutes. |
| Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
