Recombinant Human PI3, His-tagged

• Cat.No.: PI3-95H
• Product Overview: Recombinant human PI3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: PI3, also known as elafin or peptidase inhibitor 3, is neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid. In phosphate buffered saline (pH7.4), 10% glycerol.
• Molecular Mass: 10.7kDa (101aa) confirmed by MALDI-TOF.8.5-13.5kDa (SDS-PAGE under reducing conditions.)
• AA Sequence: AVTGVPVKGQ DTVKGRVPFN GQDPVKGQVS VKGQDKVKAQ EPVKGPVSTK PGSCPIILIR CAMLNPPNRC LKDTDCPGIK KCCEGSCGMA CFVPQHHHHH H
• Endotoxin: < 1.0 eu per 1 microgram of protein (determined by lal
• Purity: >85% by SDS - PAGE.
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.2mg/ml (determined by BCA)

Gene Information

• Gene Name: PI3 peptidase inhibitor 3, skin-derived [ Homo sapiens ]
• Official Symbol: PI3
• Synonyms: PI3; peptidase inhibitor 3, skin-derived; protease inhibitor 3, skin derived (SKALP); elafin; cementoin; ELAFIN; ESI; SKALP; skin derived antileukoproteinase; trappin 2; WAP3; WFDC14; PI-3; trappin-2; pre-elafin; protease inhibitor WAP3; elastase-specific inhibitor; skin-derived antileukoproteinase; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; protease inhibitor 3, skin-derived (SKALP); MGC13613
• Gene ID: 5266
• mRNA Refseq: NM_002638
• Protein Refseq: NP_002629
• MIM: 182257
• UniProt ID: P19957
• Chromosome Location: 20q13.12
• Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity