Active Recombinant Human NOTCH1 protein, Fc-tagged

• Cat.No.: NOTCH1-384H
• Product Overview: Recombinant Human NOTCH1(Ala19 - Gln526) fused with Fc of Human IgG1 at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ala19
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rhNotch-1 Fc Chimera at 5 µg/mL (100 µL/well) can bind rrJagged-1/Fc Chimera with an apparent KD <5 nM in a functional ELISA.
• Molecular Mass: Predicted Molecular Mass: 80.1 (monomer) kDa;SDS-PAGE: 115-125 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: NOTCH1 notch 1 [ Homo sapiens ]
• Official Symbol: NOTCH1
• Synonyms: NOTCH1; notch 1; Notch (Drosophila) homolog 1 (translocation associated) , Notch homolog 1, translocation associated (Drosophila) , TAN1; neurogenic locus notch homolog protein 1; Notch homolog 1, translocation-associated; translocation-associated notch protein TAN-1; hN1; TAN1
• Gene ID: 4851
• mRNA Refseq: NM_017617
• Protein Refseq: NP_060087
• MIM: 190198
• UniProt ID: P46531
• Chromosome Location: 9q34.3
• Pathway: Activated NOTCH1 Transmits Signal to the Nucleus, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Dorso-ventral axis formation, organism-specific biosystem; Dorso-ventral axis formation, conserved biosystem
• Function: calcium ion binding; chromatin DNA binding; core promoter binding; protein binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity