Active Recombinant Rat Csf1

• Cat.No.: Csf1-13R
• Product Overview: MCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254). M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains.

Specification

• Description: Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
• Source: HEK293
• Species: Rat
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. The recombinant MCSF was lyophilized after extensive dialysis against PBS.
• Bio-activity: The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Stability: Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18ºC. Upon reconstitution MCSF should be stored at 4ºC between 2-7 days and for future use below -18ºC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the MCSF in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Csf1
• Synonyms: Csf1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; CSF-1; MCSF; NP_076471.3; EC 2.7.10.1
• Gene ID: 78965
• mRNA Refseq: NM_023981
• Protein Refseq: NP_076471
• UniProt ID: Q8JZQ0
• Chromosome Location: 2q34
• Pathway: Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response (BioCarta); Hematopoietic cell lineage
• Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding