"CD55" Related Products


Recombinant Human CD55, His-tagged

Cat.No.: CD55-65H
Product Overview: Recombinant Human CD55 /DAF Protein (rhCD55) Asp 35 - Ser 353 was produced in human 293 cells (HEK293).
Description: This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Source: HEK293
Species: Human
Tag: His
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity: Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 μg/ml (100 μl/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15­-2.0 μg/ml.
Molecular Mass: rhCD55, fused with 6×His tag at the C-terminus, has a calculated MW of 35.8 kDa. The predicted N-terminus is Asp 35 . DTT-reduced Protein migrates as 50-65 kDa due to glycosylation.
Endotoxin: Less than 1.0 EU per μg of the rhCD55 by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC-8ºC); After reconstitution under sterile conditions for 1 month (4ºC-8ºC) or 3 months (-20ºC to -70ºC).
Reconstitution: See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Gene Name: CD55 CD55 molecule, decay accelerating factor for complement (Cromer blood group) [ Homo sapiens (human) ]
Official Symbol: CD55
Synonyms: CD55; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CR; TC; DAF; CROM; complement decay-accelerating factor; CD55 antigen
Gene ID: 1604
mRNA Refseq: NM_000574
Protein Refseq: NP_000565
MIM: 125240
UniProt ID: P08174
Chromosome Location: 1q32
Pathway: Class B/2 (Secretin family receptors); Complement Activation, Classical Pathway; Complement and coagulation cascades
Function: lipid binding; protein binding; virus receptor activity

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