Recombinant Human FLT3, Fc Chimera

Cat.No.: FLT3-155H
Product Overview: Recombinant Human FLT3 (Accession # AAA18947) was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: Fc Chimera
Predicted N Terminal: Asn27
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro-B cells transfected with Flt-3.The ED50 for this effect is typically 0.01 - 0.03 µg/mL in the presence of rhFlt-3 Ligand at 2 ng/mL.
Molecular Mass: Recombinant Human FLT3, Fc Chimera has a calculated MW of 85.5 kDa (monomer). In SDS-PAGE migrates as 120 kDa, reducing conditions.
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
Official Symbol: FLT3
Synonyms: FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III; FLK-2;
Gene ID: 2322
mRNA Refseq: NM_004119
Protein Refseq: NP_004110
MIM: 136351
UniProt ID: P36888
Chromosome Location: 13q12

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