Active Recombinant Human CASP10, His-tagged

• Cat.No.: CASP10-247H
• Product Overview: Recombinant Human CASP10 (Accession # AAC50644) Val220-Ile479, fused with a C-terminal 6-His tag, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Val220 & Ala373
• Form: Supplied as a 0.2 µm filtered solution in HEPES, NaCl and Sucrose.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate N-acetyl-Leu-Glu-His-Asp-7-amino-4-trifluoromethyl coumarin (Ac-LEHD-AFC).The specific activity is >200 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CASP10, His-tagged has a calculated MW of 30 kDa. In SDS-PAGE migrates as 17 kDa and 10 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CASP10 caspase 10, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Official Symbol: CASP10
• Synonyms: CASP10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis related cysteine protease; caspase-10; MCH4; CASP-10; FADD-like ICE2; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2; ALPS2; FLICE2
• Gene ID: 843
• mRNA Refseq: NM_001206524
• Protein Refseq: NP_001193453
• MIM: 601762
• UniProt ID: Q92851
• Chromosome Location: 2q33-q34