Active Recombinant Mouse Bid

• Cat.No.: Bid-258M
• Product Overview: Recombinant Mouse Bid (Accession # AAC71064) Met1-Asp195 was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Mouse
• Predicted N Terminal: Met1
• Form: Supplied as a 0.2 µm filtered solution in HEPES and KCl.
• Bio-activity: Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.The typical EC50 for cytochrome c releasing activity is less than 300 nM. Cytochrome c is quantified using the Rat/Mouse Cytochrome c Quantikine® ELISA Kit. The EC50 for the desired application should be determined. Recombinant Mouse BID Caspase-8-cleaved is available.
• Molecular Mass: Recombinant Mouse Bid has a calculated MW of 22 kDa. In SDS-PAGE migrates as 22 kDa, under reducing and non-reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Bid BH3 interacting domain death agonist [ Mus musculus ]
• Official Symbol: Bid
• Synonyms: BID; BH3 interacting domain death agonist; BH3-interacting domain death agonist; p22 BID; AI875481; AU022477; 2700049M22Rik
• Gene ID: 12122
• mRNA Refseq: NM_007544
• Protein Refseq: NP_031570