Recombinant Human IBSP, His-tagged


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Recombinant Human IBSP, His-tagged

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Cat.No. : IBSP-261H
Product Overview : Recombinant Human IBSP (Accession # AAC95490) Phe17-Gln317, fused with a C-terminal 10-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Human
Tag : His
Predicted N Terminal : Phe17
Form : Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.When 5 x10 4 cells/well are added to recombinant human IBSP-coated plates (3 µg/mL with 100 µL/well), approximately 60 - 80% will adhere after 30 minutes at 37° C.Optimal concentration depends on cell type as well as the application or research objectives.
Molecular Mass : Recombinant Human IBSP, His-tagged has a calculated MW of 34.7 kDa. In SDS-PAGE migrates as 75-90 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : IBSP integrin-binding sialoprotein [ Homo sapiens ]
Official Symbol : IBSP
Synonyms : IBSP; integrin-binding sialoprotein; bone sialoprotein 2; bone sialoprotein; bone sialoprotein II; BSP; BSP II; SP II; cell-binding sialoprotein; BNSP; SP-II; BSP-II;
Gene ID : 3381
mRNA Refseq : NM_004967
Protein Refseq : NP_004958
MIM : 147563
UniProt ID : P21815
Chromosome Location : 4q21.1
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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