Active Recombinant Human IBSP, His-tagged

• Cat.No.: IBSP-261H
• Product Overview: Recombinant Human IBSP (Accession # AAC95490) Phe17-Gln317, fused with a C-terminal 10-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: Phe17
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.When 5 x10 4 cells/well are added to recombinant human IBSP-coated plates (3 µg/mL with 100 µL/well), approximately 60 - 80% will adhere after 30 minutes at 37° C.Optimal concentration depends on cell type as well as the application or research objectives.
• Molecular Mass: Recombinant Human IBSP, His-tagged has a calculated MW of 34.7 kDa. In SDS-PAGE migrates as 75-90 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IBSP integrin-binding sialoprotein [ Homo sapiens ]
• Official Symbol: IBSP
• Synonyms: IBSP; integrin-binding sialoprotein; bone sialoprotein 2; bone sialoprotein; bone sialoprotein II; BSP; BSP II; SP II; cell-binding sialoprotein; BNSP; SP-II; BSP-II
• Gene ID: 3381
• mRNA Refseq: NM_004967
• Protein Refseq: NP_004958
• MIM: 147563
• UniProt ID: P21815
• Chromosome Location: 4q21.1