Active Recombinant Canine VEGFA

• Cat.No.: VEGFA-270C
• Product Overview: Recombinant Canine VEGFA (Accession # AAD29682) Pro28-Arg190 was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Canine
• Predicted N Terminal: Pro28
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 1.5 - 7.5 ng/mL.
• Molecular Mass: Recombinant Canine VEGFA has a calculated MW of 19 kDa (monomer).
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Canis lupus familiaris (dog) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; vascular endothelial growth factor 188
• Gene ID: 403802
• mRNA Refseq: NM_001003175
• Protein Refseq: NP_001003175
• UniProt ID: Q9MYV3
• Chromosome Location: 12