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Recombinant Mouse Bche, His-tagged

Cat.No.: Bche-355M
Product Overview: Recombinant Mouse Bche (Accession # AAH99977) His28-Leu603, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Mouse
Tag: His
Predicted N Terminal: His28
Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity: Measured by its ability to cleave Butyrylthiocholine.The specific activity is >75 nmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Mouse Bche, His-tagged has a calculated MW of 66 kDa. In SDS-PAGE migrates as 86 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Bche butyrylcholinesterase [ Mus musculus ]
Official Symbol: Bche
Synonyms: BCHE; butyrylcholinesterase; cholinesterase; choline esterase II; pseudocholinesterase; butyrylcholine esterase; acylcholine acylhydrolase; C730038G20Rik; MGC107651;
Gene ID: 12038
mRNA Refseq: NM_009738
Protein Refseq: NP_033868
MIM:

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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