||Recombinant Human IL32 (Accession # AAS80146) Cys2-Lys131 was produced in E. coli-derived.
|Predicted N Terminal:
||Lyophilized from a 0.2 µm filtered solution in PBS and DTT.
||Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cellsunder serum free conditions in the presence of muramyl dipeptide (MDP) and Polymyxin B. Netea, M.G. et al. (2005) Proc. Nat. Acad. Sci. 102:16309.The ED50 for this effect is typically 2 - 12 µg/mL.
||Recombinant Human IL32 has a calculated MW of 14.9 kDa. In SDS-PAGE migrates as 18.8 kDa, reducing conditions.
||>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).