||Recombinant Human SRC (Accession # NM_005417) , fused with an N-terminal GST tag, was produced in E. coli.
||Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
||The specific activity of Src was determined to be 100 nmol/min/mg using a poly (Glu:Tyr, 4:1) synthetic peptide substrate
||In SDS-PAGE migrates as 83 kDa.
||The purity was determined to be >95% by densitometry
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).