"INS" Related Products

Recombinant Human INS, Met, His&Lys-tagged

Cat.No.: INS-607H
Product Overview: Recombinant Human INS (Accession # NP_000198) Phe25-Asn110, fused with an N-terminal Met, 6-His tag and Lys, was produced in E. coli-derived.
Source: E. coli
Species: Human
Tag: , His&Lys
Predicted N Terminal: Met
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.15 - 0.75 µg/mL.
Molecular Mass: Recombinant Human INS, Met, His&Lys-tagged has a calculated MW of 10.5 kDa. In SDS-PAGE migrates as 9 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: INS insulin [ Homo sapiens ]
Official Symbol: INS
Synonyms: INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10;
Gene ID: 3630
mRNA Refseq: NM_000207
Protein Refseq: NP_000198
MIM:
UniProt ID: P01308
Chromosome Location: 11p15.5

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