Recombinant Human INS, Met, His&Lys-tagged INS-607H

Recombinant Human INS, Met, His&Lys-tagged


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Recombinant Human INS, Met, His&Lys-tagged

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Cat.No. : INS-607H
Product Overview : Recombinant Human INS (Accession # NP_000198) Phe25-Asn110, fused with an N-terminal Met, 6-His tag and Lys, was produced in E. coli-derived.
Source : E. coli
Species : Human
Tag : , His&Lys
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.15 - 0.75 µg/mL.
Molecular Mass : Recombinant Human INS, Met, His&Lys-tagged has a calculated MW of 10.5 kDa. In SDS-PAGE migrates as 9 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : INS insulin [ Homo sapiens ]
Official Symbol : INS
Synonyms : INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10;
Gene ID : 3630
mRNA Refseq : NM_000207
Protein Refseq : NP_000198
UniProt ID : P01308
Chromosome Location : 11p15.5

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