||Recombinant Feline VEGFA (Accession # NP_001009854) Ala27-Arg189, fused with an N-terminal Met, was produced in E. coli-derived.
|Predicted N Terminal:
||Met & Pro28
||Lyophilized from a 0.2 µm filtered solution in HCl.
||Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 1.2 - 6 ng/mL.
||Recombinant Feline VEGFA, Met-tagged has a calculated MW of 19.2 kDa (monomer). In SDS-PAGE migrates as 20 kDa, reducing conditions.
||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).