||Recombinant Human IL32 (Accession # NP_001012649) was produced in E. coli-derived.
|Predicted N Terminal:
||Supplied as a 0.2 µm filtered solution in HEPES, NaCl, DTT and CHAPS.
||Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells under serum free conditions in the presence of 10 µg/mL of Polymyxin B. Kim, S.H. et al. (2005) Immunity 22:131.The ED50 for this effect is typically 10-50 ng/mL.
||Recombinant Human IL32 has a calculated MW of 23.1 kDa. In SDS-PAGE migrates as 28 kDa & some aggregates, reducing conditions.
||>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).