Active Recombinant Human VEGFB protein, hFc-tagged

• Cat.No.: VEGFB-275H
• Product Overview: Recombinant Human VEGFB protein(NP_003368.1)(Pro22-Ala207), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized human S4-Fc3L3-VEGFB at 10 μg/ml (100 μL/well) can bind biotinylated human NRP1-Fc, The EC50 of biotinylated human NRP1-Fc is 0.03-0.07μg/mL.
• Molecular Mass: The recombinant human VEGFB consists 446 amino acids and predicts a molecular mass of 47.8 kDa.
• Proteinlength: Pro22-Ala207
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFB vascular endothelial growth factor B [ Homo sapiens ]
• Official Symbol: VEGFB
• Synonyms: VEGFB; vascular endothelial growth factor B; VRF; VEGFL; VEGF-related factor
• Gene ID: 7423
• mRNA Refseq: NM_001243733
• Protein Refseq: NP_001230662
• MIM: 601398
• UniProt ID: P49765