Active Recombinant Human SPARC Protein, His-tagged
Cat.No. : | SPARC-525H |
Product Overview : | Recombinant Human SPARC (Accession # NP_003109) Ala18-Ile303, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
- Specification
- Gene Information
- Related Products
Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Ala18 |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS. |
Bio-activity : | Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977. The ED50 for this effect is typically 0.75 - 3.0 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhSPARC with an apparent KD < 40 nm. Optimal dilutions should be determined by each laboratory for each application. |
Molecular Mass : | Recombinant Human SPARC, His-tagged has a calculated MW of 34 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions. |
Protein length : | Ala18-Ile303 |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Gene Name : | SPARC secreted protein, acidic, cysteine-rich (osteonectin) [ Homo sapiens ] |
Official Symbol : | SPARC |
Synonyms : | SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine rich protein; osteonectin; BM-40; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine; |
Gene ID : | 6678 |
mRNA Refseq : | NM_003118 |
Protein Refseq : | NP_003109 |
MIM : | 182120 |
UniProt ID : | P09486 |
Chromosome Location : | 5q31-q33 |
Products Types
◆ Recombinant Protein | ||
SPARC-001H | Recombinant Human SPARC Protein, MBP-tagged | +Inquiry |
SPARC-5342R | Recombinant Rat SPARC Protein, His (Fc)-Avi-tagged | +Inquiry |
Sparc-010M | Recombinant Mouse Sparc Protein, His-tagged | +Inquiry |
Sparc-8608M | Recombinant Mouse Sparc Protein, His (Fc)-Avi-tagged | +Inquiry |
SPARC-2725H | Recombinant Human SPARC Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
SPARC-287B | Native Bovine Osteonectin | +Inquiry |
SPARC-30653TH | Native Human SPARC | +Inquiry |
◆ Lysates | ||
SPARC-2120MCL | Recombinant Mouse SPARC cell lysate | +Inquiry |
SPARC-2616HCL | Recombinant Human SPARC cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionSPARC is associated with cancer progression, as it can influence tumor growth, invasion, and angiogenesis. Its expression levels in tumors are often studied for prognostic and diagnostic purposes.
Yes, SPARC is being explored as a therapeutic target for cancer. Researchers are investigating ways to modulate its activity to hinder tumor progression or enhance the efficacy of existing treatments.
SPARC expression can be detected through techniques like immunohistochemistry, Western blotting, and molecular assays. These methods help assess SPARC levels in tissues or blood samples.
SPARC plays a role in tissue repair by influencing cell-matrix interactions and modulating the extracellular environment. Its involvement in wound healing and tissue regeneration makes it a subject of interest in regenerative medicine.
Yes, there are clinical trials exploring the potential of targeting SPARC in various diseases, including cancer. These trials aim to evaluate the safety and efficacy of SPARC-modulating agents.
Customer Reviews (3)
Write a reviewThe SPARC protein finds great utility in protein electron microscopy structure analysis, consistently delivering exceptional results.
Its remarkable sensitivity and specificity make it a valuable tool for accurate and robust data analysis, bolstering the credibility of research outcomes.
Notably, it exhibits exceptional performance in ELISA assays, providing reliable and precise results in the detection and quantification of target molecules.
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