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Active Recombinant Human SPARC Protein, His-tagged

Cat.No. : SPARC-525H
Product Overview : Recombinant Human SPARC (Accession # NP_003109) Ala18-Ile303, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
  • Specification
  • Gene Information
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Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Ala18
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977. The ED50 for this effect is typically 0.75 - 3.0 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhSPARC with an apparent KD < 40 nm. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Human SPARC, His-tagged has a calculated MW of 34 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions.
Protein length : Ala18-Ile303
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : SPARC secreted protein, acidic, cysteine-rich (osteonectin) [ Homo sapiens ]
Official Symbol : SPARC
Synonyms : SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine rich protein; osteonectin; BM-40; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine;
Gene ID : 6678
mRNA Refseq : NM_003118
Protein Refseq : NP_003109
MIM : 182120
UniProt ID : P09486
Chromosome Location : 5q31-q33

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is the SPARC protein linked to cancer? 04/18/2023

SPARC is associated with cancer progression, as it can influence tumor growth, invasion, and angiogenesis. Its expression levels in tumors are often studied for prognostic and diagnostic purposes.

Can SPARC be a potential therapeutic target for cancer treatment? 12/08/2022

Yes, SPARC is being explored as a therapeutic target for cancer. Researchers are investigating ways to modulate its activity to hinder tumor progression or enhance the efficacy of existing treatments.

In what ways can SPARC expression be detected and measured in clinical settings? 04/25/2021

SPARC expression can be detected through techniques like immunohistochemistry, Western blotting, and molecular assays. These methods help assess SPARC levels in tissues or blood samples.

How does SPARC contribute to tissue repair and regeneration? 03/04/2021

SPARC plays a role in tissue repair by influencing cell-matrix interactions and modulating the extracellular environment. Its involvement in wound healing and tissue regeneration makes it a subject of interest in regenerative medicine.

Are there any ongoing clinical trials targeting SPARC for therapeutic interventions? 03/28/2019

Yes, there are clinical trials exploring the potential of targeting SPARC in various diseases, including cancer. These trials aim to evaluate the safety and efficacy of SPARC-modulating agents.

Customer Reviews (3)

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Reviews
07/18/2021

    The SPARC protein finds great utility in protein electron microscopy structure analysis, consistently delivering exceptional results.

    02/09/2017

      Its remarkable sensitivity and specificity make it a valuable tool for accurate and robust data analysis, bolstering the credibility of research outcomes.

      07/10/2016

        Notably, it exhibits exceptional performance in ELISA assays, providing reliable and precise results in the detection and quantification of target molecules.

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