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Recombinant Human SPARC Protein, His-tagged

Cat.No.: SPARC-525H
Product Overview: Recombinant Human SPARC (Accession # NP_003109) Ala18-Ile303, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Human
Tag: His
Predicted N Terminal: Ala18
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977. The ED50 for this effect is typically 0.75 - 3.0 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhSPARC with an apparent KD < 40 nm. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass: Recombinant Human SPARC, His-tagged has a calculated MW of 34 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions.
Protein length: Ala18-Ile303
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: SPARC secreted protein, acidic, cysteine-rich (osteonectin) [ Homo sapiens ]
Official Symbol: SPARC
Synonyms: SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine rich protein; osteonectin; BM-40; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine;
Gene ID: 6678
mRNA Refseq: NM_003118
Protein Refseq: NP_003109
MIM: 182120
UniProt ID: P09486
Chromosome Location: 5q31-q33

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