Recombinant Mouse Ibsp Protein, His-tagged Ibsp-600M

Recombinant Mouse Ibsp Protein, His-tagged

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Recombinant Mouse Ibsp Protein, His-tagged

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Cat.No. : Ibsp-600M
Product Overview : Recombinant Mouse Ibsp (Accession # NP_032344) Met1-Gln324, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Mouse
Tag : His
Predicted N Terminal : Phe17
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.When 5 x 10^4 cells/well are added to recombinant mouse IBSP coated plates (3 µg/mL with 100 µL/well), approximately 60 - 80% will adhere after 30 minutes at 37 °C.Optimal concentration depends on cell type as well as the application or research objectives.
Molecular Mass : Recombinant Mouse Ibsp, His-tagged has a calculated MW of 34.9 kDa. In SDS-PAGE migrates as 70-90 kDa, reducing conditions.
Protein length : Met1-Gln324
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Ibsp integrin binding sialoprotein [ Mus musculus ]
Official Symbol : Ibsp
Synonyms : IBSP; integrin binding sialoprotein; bone sialoprotein 2; bone sialoprotein II; cell-binding sialoprotein; integrin-binding sialoprotein; BSP; Bsp2; BSPII; BSP II;
Gene ID : 15891
mRNA Refseq : NM_008318
Protein Refseq : NP_032344
MIM :

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