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Active Recombinant Mouse Il1b

Cat.No. : Il1b-603M
Product Overview : Recombinant Mouse Il1b (Accession # NP_032387) Val118-Ser269, fused with an N-terminal Met, was produced in E. coli.
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  • Gene Information
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Source : E. coli
Species : Mouse
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.The ED50 for this effect is typically 2-10 pg/mL.
Molecular Mass : Recombinant Mouse Il1b, Met-tagged has a calculated MW of 17 kDa.
Protein length : Val118-Ser269
Purity : >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Il1b interleukin 1 beta [ Mus musculus ]
Official Symbol : Il1b
Synonyms : IL1B; interleukin 1 beta; interleukin-1 beta; IL-1 beta; Il-1b; IL-1beta;
Gene ID : 16176
mRNA Refseq : NM_008361
Protein Refseq : NP_032387
MIM :

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does IL-1β initiate inflammasome assembly, and which experimental techniques unravel this intricate process? 07/30/2022

Inflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.

What are the specific mechanisms underlying IL-1β's priming of immune cells, and how can flow cytometry and gene expression analyses decipher these molecular pathways? 07/08/2021

IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.

How do post-translational modifications regulate IL-1β's biological activity, and how can mass spectrometry-based phosphoproteomics provide insights into these modifications? 06/06/2020

Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.

How can advanced bioinformatics tools be applied to multi-omics data to comprehensively understand the intricate roles of IL-1β in health and disease? 04/16/2020

Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.

What is the significance of IL-1β's epigenetic regulation in chronic inflammatory diseases, and how can ChIP-seq and DNA methylation profiling uncover these regulatory elements? 11/01/2019

ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.

How does IL-1β interact with its downstream effectors to induce the production of pro-inflammatory cytokines, and how can dual-luciferase reporter assays reveal these intricate interactions? 03/31/2019

IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.

What are the complex feedback loops between IL-1β and other cytokines, and how can systems biology approaches like network analysis dissect these dynamic interactions? 01/24/2019

Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.

What is the role of IL-1β in modulating the tumor microenvironment, and how can advanced imaging techniques like intravital microscopy elucidate its impact on immune cell dynamics? 08/08/2017

IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.

What are the mechanisms underlying IL-1β's non-canonical signaling pathways, and how can CRISPR-Cas9 screening identify key mediators of these alternative pathways? 04/18/2017

CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.

How does IL-1β's signaling adapt to different cellular contexts, and how can single-cell RNA sequencing uncover cell-specific responses to this cytokine? 01/03/2017

Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.

Customer Reviews (3)

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Reviews
11/08/2021

    Effective in inflammation studies.

    04/23/2021

      High specificity.

      06/30/2020

        Suitable for cytokine assays.

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