Recombinant Human SPHK1 Protein, Met & His-tagged SPHK1-741H

Recombinant Human SPHK1 Protein, Met & His-tagged

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Recombinant Human SPHK1 Protein, Met & His-tagged

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Cat.No. : SPHK1-741H
Product Overview : Recombinant Human SPHK1 (Accession # NP_068807) Asp2-Leu398, fused with an N-terminal Met and 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source : Baculovirus Sf21 Insect Cells
Species : Human
Tag : & His
Predicted N Terminal : Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Form : Supplied as a 0.2 µm filtered solution in MES, NaCl, Glycerol and DTT.
Bio-activity : Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine).The specific activity is >200 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human SPHK1, Met & His-tagged has a calculated MW of 45 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions.
Protein length : Asp2-Leu398
Purity : >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : SPHK1 sphingosine kinase 1 [ Homo sapiens ]
Official Symbol : SPHK1
Synonyms : SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1;
Gene ID : 8877
mRNA Refseq : NM_001142601
Protein Refseq : NP_001136073
MIM : 603730
UniProt ID : Q9NYA1
Chromosome Location : 17q25.2

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