Active Recombinant Human SPHK1 Protein, Met & His-tagged
Cat.No. : | SPHK1-741H |
Product Overview : | Recombinant Human SPHK1 (Accession # NP_068807) Asp2-Leu398, fused with an N-terminal Met and 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived. |
- Specification
- Gene Information
- Related Products
Source : | Baculovirus Sf21 Insect Cells |
Species : | Human |
Tag : | & His |
Predicted N Terminal : | Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments. |
Form : | Supplied as a 0.2 µm filtered solution in MES, NaCl, Glycerol and DTT. |
Bio-activity : | Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine).The specific activity is >200 pmol/min/µg, as measured under the described conditions. |
Molecular Mass : | Recombinant Human SPHK1, Met & His-tagged has a calculated MW of 45 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions. |
Protein length : | Asp2-Leu398 |
Purity : | >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Gene Name : | SPHK1 sphingosine kinase 1 [ Homo sapiens ] |
Official Symbol : | SPHK1 |
Synonyms : | SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1; |
Gene ID : | 8877 |
mRNA Refseq : | NM_001142601 |
Protein Refseq : | NP_001136073 |
MIM : | 603730 |
UniProt ID : | Q9NYA1 |
Chromosome Location : | 17q25.2 |
Products Types
◆ Recombinant Protein | ||
SPHK1-4130H | Recombinant Human SPHK1 Protein, His (Fc)-Avi-tagged | +Inquiry |
SPHK1-0269H | Recombinant Human SPHK1 Protein (M1-S363), Tag Free | +Inquiry |
SPHK1-012H | Recombinant Human SPHK1 Protein, His-tagged | +Inquiry |
SPHK1-2567H | Recombinant Human SPHK1 Protein, MYC/DDK-tagged | +Inquiry |
SPHK1-12H | Recombinant Human SPHK1 Protein, N-His-tagged | +Inquiry |
◆ Lysates | ||
SPHK1-001HCL | Recombinant Human SPHK1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionElevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.
SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.
Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.
SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.
Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.
Customer Reviews (3)
Write a reviewIts seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.
With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.
Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.
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