Recombinant Mouse Sphingosine Kinase 1, His-tagged

Cat.No. : Sphk1-524M
Product Overview : Recombinant Mouse Sphingosine kinase 1 (full length) was expressed in a Baculovirus infected Sf9 cell expression system with N-terminal His tag. MW=47.10 kDa.
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Cat. No. : Sphk1-524M
Description : Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1. Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor and serum.
Source : Baculovirus infected Sf9 cells.
Formulated In : 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Application : useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity : 20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 4 uM D-erythrosphingosine, and 0.1-0.5 ug/ml sphingosine kinase 1.
Purity : >50% by SDS-PAGE.
Stability : > 6 months at –80°C.
Pathways : Calcium signaling pathway; Fc gamma R-mediated phagocytosis; Metabolic pathways; Sphingolipid metabolism; VEGF signaling pathway
Gene Name : Sphk1 sphingosine kinase 1 [ Mus musculus ]
Synonyms : sphingosine kinase 1; SK1; Sphk1; 1110006G24Rik; OTTMUSP00000004201; OTTMUSP00000004202; OTTMUSP00000004203; OTTMUSP00000004204; OTTMUSP00000004205
Gene ID : 20698
mRNA Refseq : NM_011451
Protein Refseq : NP_035581
UniProt ID : Q8CI15
Chromosome Location : 11 E2
Function : ATP binding; DNA binding; calmodulin binding; diacylglycerol kinase activity; kinase activity; nucleotide binding; sphinganine kinase activity; transferase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does SPHK1 contribute to drug resistance in cancer? 10/09/2021

Elevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.

How does SPHK1 contribute to immune regulation? 08/03/2021

SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.

How might the modulation of SPHK1 impact personalized medicine? 04/06/2018

Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.

How does SPHK1 influence vascular functions? 01/11/2017

SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.

Can SPHK1 inhibitors be used in combination therapies? 04/24/2016

Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.

Customer Reviews (3)

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04/16/2023

    Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

    04/14/2020

      With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

      01/03/2020

        Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

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