Active Recombinant Mouse F11 Protein, His-tagged

• Cat.No.: F11-766M
• Product Overview: Recombinant Mouse F11 (Accession # NP_082342) Glu19-Val624, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Glu19
• Form: Supplied as a 0.2 µm filtered solution in HEPES and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse F11, His-tagged has a calculated MW of 69 kDa. In SDS-PAGE migrates as 74 kDa, reducing conditions.
• Protein length: Glu19-Val624
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: F11 coagulation factor XI [ Mus musculus ]
• Official Symbol: F11
• Synonyms: F11; coagulation factor XI; PTA; plasma thromboplastin antecedent; FXI; Cf11; AI503996; 1600027G01Rik
• Gene ID: 109821
• mRNA Refseq: NM_028066
• Protein Refseq: NP_082342