Active Recombinant Mouse Rspo2

• Cat.No.: Rspo2-850M
• Product Overview: Recombinant Mouse Rspo2 (Accession # NP_766403) Met1-Gly205, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Predicted N Terminal: Predicted Gln22, not observed that sequencing might be blocked & Ala32
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically 0.4-2.4 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.
• Molecular Mass: Recombinant Mouse Rspo2 has a calculated MW of 21 kDa & 20 kDa. In SDS-PAGE migrates as 26-27 kDa, reducing conditions.
• Protein length: Met1-Gly205
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.

Gene Information

• Gene Name: Rspo2 R-spondin 2 homolog (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Rspo2
• Synonyms: RSPO2; R-spondin 2 homolog (Xenopus laevis); R-spondin-2; cristin-2; mCristin-2; roof plate-specific spondin-2; cysteine-rich and single thrombospondin domain-containing protein 2; ftls; AA673245; D430027K22; 2610028F08Rik
• Gene ID: 239405
• mRNA Refseq: NM_172815
• Protein Refseq: NP_766403