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Recombinant Mouse Mstn Protein, His-tagged

Cat.No.: Mstn-910M
Product Overview: Recombinant Mouse Mstn (Accession # O08689) Asn25-Ser265, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Mouse
Tag: His
Predicted N Terminal: Asn25
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Thies, R.S. et al. (2001) Growth Factors 18:251.The ED50 for this effect is typically 0.05-0.25 µg/mL in the presence of 40 ng/mL of Recombinant Mouse GDF-8/Myostatin. Approximately 1 µg/mL will completely inhibit GDF-8 activity in these cells.
Molecular Mass: Recombinant Mouse Mstn, His-tagged has a calculated MW of 28.7 kDa. In SDS-PAGE migrates as 39 kDa, reducing conditions.
Protein length: Asn25-Ser265
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Mstn myostatin [ Mus musculus ]
Official Symbol: Mstn
Synonyms: MSTN; myostatin; growth/differentiation factor 8; GDF-8; compact; growth differentiation factor 8; Cmpt; Gdf8; MGC124261; MGC124262; MGC124263;
Gene ID: 17700
mRNA Refseq: NM_010834
Protein Refseq: NP_034964
MIM:

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