Active Recombinant Mouse Mstn Protein, His-tagged

• Cat.No.: Mstn-910M
• Product Overview: Recombinant Mouse Mstn (Accession # O08689) Asn25-Ser265, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Asn25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Thies, R.S. et al. (2001) Growth Factors 18:251.The ED50 for this effect is typically 0.05-0.25 µg/mL in the presence of 40 ng/mL of Recombinant Mouse GDF-8/Myostatin. Approximately 1 µg/mL will completely inhibit GDF-8 activity in these cells.
• Molecular Mass: Recombinant Mouse Mstn, His-tagged has a calculated MW of 28.7 kDa. In SDS-PAGE migrates as 39 kDa, reducing conditions.
• Protein length: Asn25-Ser265
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Mstn myostatin [ Mus musculus ]
• Official Symbol: Mstn
• Synonyms: MSTN; myostatin; growth/differentiation factor 8; GDF-8; compact; growth differentiation factor 8; Cmpt; Gdf8; MGC124261; MGC124262; MGC124263
• Gene ID: 17700
• mRNA Refseq: NM_010834
• Protein Refseq: NP_034964