Active Recombinant Human CA12 Protein, His-tagged

• Cat.No.: CA12-971H
• Product Overview: Recombinant Human CA12 (Accession # O43570) Ala25-Gln291, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala25
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its esterase activity. The specific activity is >10 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CA12, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 38-41 kDa, reducing conditions.
• Protein length: Ala25-Gln291
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CA12 carbonic anhydrase XII [ Homo sapiens ]
• Official Symbol: CA12
• Synonyms: CA12; carbonic anhydrase XII; carbonic anhydrase 12; HsT18816; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; CAXII; FLJ20151
• Gene ID: 771
• mRNA Refseq: NM_001218
• Protein Refseq: NP_001209
• MIM: 603263
• UniProt ID: O43570
• Chromosome Location: 15q22