||Recombinant human SDF-1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 10.8 kDa (93aa)
||SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). SDF-1 is strongly chemotactic for lymphocytes andworks by its receptor CXCR4. The SDF-1+CXCR4 complex plays a significant role in the creation of metastases of neoplasms and as a response to cytostatic treatment. Identification of this complex may be a useful prognostic factor in the therapy of many types of carcinoma.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKR FKM
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
||< 1.0 EU per 1 μg of protein (determined by LAL method )
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.