Active Recombinant Human ADA protein, His-tagged

• Cat.No.: ADA-1112H
• Product Overview: Recombinant Human ADA(Met1-Leu363) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-363 a.a.
• Description: Adenosine Deaminase (ADA, adenosine aminohydrolase) is one of the key enzymes of purine nucleotide catabolism. It catalyses the hydrolytic deamination of adenosine and deoxy-adenosine to inosine and deoxyinosine. ADA is expressed in virtually all tissues and is expressed at high levels in T-lymphocytes. Adenosine Deaminase deficiency can cause a form of SCID (severe combined immunodeficiency) and lymphopenia in both B- and T-cell lineages. ADA can be used as a sensitive diagnostic marker for tuberculous pleuritis. Although it is primarily a cytosolic enzyme, ADA is known to be a positive regulator of T-cell co-activation due to its binding to CD26 at the cell surface. The interaction of ADA with CD26 regulates lymphocyte-epithelial cell adhesion.
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: >44,000 pmol/min/μg
• Molecular Mass: Predicted Molecular Mass: 42 kDa;SDS-PAGE: 40-42 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions
• Storage: Avoid repeated freeze-thaw cycles.3 months from date of receipt, -70 centigrade as supplied.1 month, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: ADA adenosine deaminase [ Homo sapiens ]
• Official Symbol: ADA
• Synonyms: ADA; adenosine deaminase; adenosine aminohydrolase
• Gene ID: 100
• mRNA Refseq: NM_000022
• Protein Refseq: NP_000013
• MIM: 608958
• UniProt ID: P00813
• Chromosome Location: 20q13.12
• Pathway: C-MYB transcription factor network, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Primary immunodeficiency, organism-specific biosystem; Primary immunodeficiency, conserved biosystem; Purine metabolism, organism-specific biosystem
• Function: adenosine deaminase activity; adenosine deaminase activity; hydrolase activity; metal ion binding; protein binding; purine nucleoside binding; zinc ion binding; zinc ion binding