Recombinant Human USP5 protein, His-tagged

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Recombinant Human USP5 protein, His-tagged

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Cat.No. : USP5-158H
Product Overview : Recombinant Human USP5(aa 1-858) fused with His tag was expressed in E. coli.
Source : E. coli
Species : Human
Tag : His
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : Deubiquitylating Enzyme Assay: The activity of His-USP5 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-USP5 was compared confirming the deubiquitylating activity of His-USP5.
Molecular Mass : ~98 kDa
Stability : 12 months at -70 centigrade; aliquot as required
Concentration : 0.5 mg/ml
Gene Name : USP5 ubiquitin specific peptidase 5 (isopeptidase T) [ Homo sapiens ]
Official Symbol : USP5
Synonyms : USP5; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5 (isopeptidase T); ubiquitin carboxyl-terminal hydrolase 5; IsoT; isopeptidase T; ubiquitin isopeptidase T; ubiquitin thioesterase 5; deubiquitinating enzyme 5; ubiquitin thiolesterase 5; ubiquitin-specific-processing protease 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ISOT;
Gene ID : 8078
mRNA Refseq : NM_001098536
Protein Refseq : NP_001092006
MIM : 601447
UniProt ID : P45974
Chromosome Location : 12p13
Function : cysteine-type endopeptidase activity; metal ion binding; omega peptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity; zinc ion binding;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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