Active Recombinant Human USP5 protein, His-tagged

• Cat.No.: USP5-158H
• Product Overview: Recombinant Human USP5(aa 1-858) fused with His tag was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylating Enzyme Assay: The activity of His-USP5 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-USP5 was compared confirming the deubiquitylating activity of His-USP5.
• Molecular Mass: ~98 kDa
• Stability: 12 months at -70 centigrade; aliquot as required
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP5 ubiquitin specific peptidase 5 (isopeptidase T) [ Homo sapiens ]
• Official Symbol: USP5
• Synonyms: USP5; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5 (isopeptidase T); ubiquitin carboxyl-terminal hydrolase 5; IsoT; isopeptidase T; ubiquitin isopeptidase T; ubiquitin thioesterase 5; deubiquitinating enzyme 5; ubiquitin thiolesterase 5; ubiquitin-specific-processing protease 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ISOT
• Gene ID: 8078
• mRNA Refseq: NM_001098536
• Protein Refseq: NP_001092006
• MIM: 601447
• UniProt ID: P45974
• Chromosome Location: 12p13
• Function: cysteine-type endopeptidase activity; metal ion binding; omega peptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity; zinc ion binding