Active Recombinant Human Histone Deacetylase 5, GST-tagged

• Cat.No.: HDAC5-396H
• Product Overview: Full Length Human HDAC5–Class IIa, 150kD with GST tag.

Specification

• Cat. No.: HDAC5-396H
• Description: Histone deacetylase 5, also known as HDAC5, is a human gene. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HDAC5 was cloned from human brain cDNA and expressed in insect cells using a baculoviral expression system.
• Purity: Purified, active enzyme.
• Purification Procedure: Glutathione Column
• Activity: 1000 units per vial. 1 unit is defined as the amount of enzyme that will convert 1picomole per minute per ug of protein at pH8 at 30°C.
• Stability/Shelf-life: >6 months from date of shipment
• Buffer Composition: 25mM Tris pH8, 120mM NaCl, 0.05% Tween-20 & 40% Glycerol.
• Storage: –80°C
• Publication:
  Functional interaction of histone deacetylase 5 (HDAC5) and lysine-specific demethylase 1 (LSD1) promotes breast cancer progression (2017)

Gene Information

• Gene Name: HDAC5 histone deacetylase 5 [ Homo sapiens ]
• Synonyms: HDAC5; FLJ90614; HD5; NY-CO-9; HDAC5 histone deacetylase 5; histone deacetylase 5; antigen NY-CO-9; EC 3.5.1.98; KIAA0600; Antigen NY-CO-9.
• Gene ID: 10014
• mRNA Refseq: NM_001015053
• Protein Refseq: NP_001015053
• MIM: 605315
• UniProt ID: Q9UQL6
• Chromosome Location: 17q21
• Pathway: Gene Expression
• Function: histone deacetylase activity; hydrolase activity; specific transcriptional repressor activity; transcription corepressor activity; transcription factor binding