|Cat. No. :
|Product Overview :
||Recombinant Human MITF protein expressed inE.coliencodes amino acids 170-279 and has a molecular mass of 38 kDa.
||Mi. is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells.There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
||Recombinant Human MITF protein encoding a.a. 170-279 expressed inE.coli.
||Recombinant Human MITF protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
|Application And Suggested Dilutions :
||•ELISA •Inhibition Assays •Western Blotting
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 38 kDa.
|Storage & Stability :
||Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.