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Recombinant Human ATP7B

Cat.No. : ATP7B-174H
Product Overview : Recombinant Wilson Protein (ATP7B, Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein) was cloned from human cDNA with a N-terminal purification tag and expressed inE.coli. The protein consists of the all six soluble domains of the human Wilson protein (residues 1-636 swissprot accession P35670) and contains 3 additional aa (SFT) in the apoform. N-term end of the protein. MW = 67.8 KDa.
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Cat. No. : ATP7B-174H
Description : Wilson disease protein (also called ATP7B) is an ATPase that transports copper.This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least two putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
Source : E.Coli.
Purity : >90% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight of 66 kDa.
Supplied As : 1mg/ml in sodium phosphate buffer 50mM pH=7.0 , arginine 50mM, glutammic acid 50mM, DTT 2mM, EDTA 1mM, pefabloc® 50uM. The concentration is calculated from the absorbance at 280nm (ε280= 24410 M-1cm-1).
Characteriatias : To avoid precipitation handle the protein in an inert atmosphere, the product can be concentrated to a maximum of 0.3mM.
Usage : metal binding studies, kinetics, WB, SDS-page, blotting, ELISA, antibody production.
Storage : -20ºC. The protein is stable at 25ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.
Gene Name : ATP7B ATPase, Cu++ transporting, beta polypeptide [ Homo sapiens ]
Synonyms : ATP7B; ATPase,Cu++ transporting, beta polypeptide; WD; PWD; WC1; WND; ATP7B; ATPase, Cu++ transporting, beta polypeptide (Wilson disease); Wilson disease;Copper-transporting ATPase 2;EC3.6.3.4; Copper pump 2; Wilson; disease-associated protein; wn/140KDa; OTTHUMP00000040880; ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide (Wilson disease); Wilson disease-associated protein
Gene ID : 540
mRNA Refseq : NM_000053
Protein Refseq : NP_000044
MIM : 606882
UniProt ID : P35670
Chromosome Location : 13q14.3
Function : ATP binding;copper ion binding;copper-exporting ATPase activity;copper-exporting ATPase activity;hydrolase activity;hydrolase activity, acting on acid anhydrides, catalyzing transmembrane;movement of substances;magnesium ion binding;metal ion transmembrane transporter activity;nucleotide binding;protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does the ATP7B protein contribute to copper transport within cells, and what cellular compartments are involved in this process? 01/14/2022

ATP7B facilitates copper transport from the cytoplasm to the Golgi apparatus, where copper is incorporated into proteins. It also mediates copper export from cells when copper levels are elevated, preventing toxicity.

How does ATP7B interact with other proteins involved in copper metabolism, and what are the implications of disruptions in these interactions? 02/22/2021

ATP7B interacts with various proteins in the copper transport pathway. Disruptions in these interactions can lead to mislocalization of ATP7B and compromise its ability to regulate copper levels properly.

How does ATP7B-mediated copper transport impact the activity of copper-dependent enzymes, and what are the broader implications for cellular function? 09/18/2020

ATP7B ensures the proper supply of copper to enzymes involved in various cellular processes, including energy production and antioxidant defense. Dysregulation can disrupt these processes, affecting cell function.

What is the role of ATP7B in copper excretion at the systemic level, and how does this relate to copper metabolism in the body? 05/30/2019

ATP7B is crucial for copper excretion into bile, a process that contributes to systemic copper balance. Dysfunctional ATP7B results in impaired copper excretion, leading to the accumulation of copper in tissues.

Can you explain the consequences of mutations in the ATP7B gene, particularly in relation to Wilson's disease? 11/05/2018

Mutations in the ATP7B gene lead to impaired copper transport, causing copper accumulation in tissues. This is associated with Wilson's disease, a disorder characterized by copper toxicity, especially in the liver and brain.

Customer Reviews (3)

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Reviews
01/15/2021

    Impressed with the product quality.

    05/09/2020

      The product exceeded expectations.

      09/28/2018

        This product consistently delivers accurate results in our experiments.

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