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Recombinant Human ATP7B

Cat. No.: ATP7B-174H
Product Overview: Recombinant Wilson Protein (ATP7B, Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein) was cloned from human cDNA with a N-terminal purification tag and expressed inE.coli. The protein consists of the all six soluble domains of the human Wilson protein (residues 1-636 swissprot accession P35670) and contains 3 additional aa (SFT) in the apoform. N-term end of the protein. MW = 67.8 KDa.
Description: Wilson disease protein (also called ATP7B) is an ATPase that transports copper.This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least two putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
Source: E.Coli.
Purity: >90% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight of 66 kDa.
Supplied As: 1mg/ml in sodium phosphate buffer 50mM pH=7.0 , arginine 50mM, glutammic acid 50mM, DTT 2mM, EDTA 1mM, pefabloc® 50uM. The concentration is calculated from the absorbance at 280nm (ε280= 24410 M-1cm-1).
Characteriatias: To avoid precipitation handle the protein in an inert atmosphere, the product can be concentrated to a maximum of 0.3mM.
Usage: metal binding studies, kinetics, WB, SDS-page, blotting, ELISA, antibody production.
Storage: -20ºC. The protein is stable at 25ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.
Gene Name: ATP7B ATPase, Cu++ transporting, beta polypeptide [ Homo sapiens ]
Synonyms: ATP7B; ATPase,Cu++ transporting, beta polypeptide; WD; PWD; WC1; WND; ATP7B; ATPase, Cu++ transporting, beta polypeptide (Wilson disease); Wilson disease;Copper-transporting ATPase 2;EC3.6.3.4; Copper pump 2; Wilson; disease-associated protein; wn/140KDa; OTTHUMP00000040880; ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide (Wilson disease); Wilson disease-associated protein
Gene ID: 540
mRNA Refseq: NM_000053
Protein Refseq: NP_000044
MIM: 606882
UniProt ID: P35670
Chromosome Location: 13q14.3
Function: ATP binding;copper ion binding;copper-exporting ATPase activity;copper-exporting ATPase activity;hydrolase activity;hydrolase activity, acting on acid anhydrides, catalyzing transmembrane;movement of substances;magnesium ion binding;metal ion transmembrane transporter activity;nucleotide binding;protein binding

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