||Recombinant Wilson Protein (ATP7B, Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein) was cloned from human cDNA with a N-terminal purification tag and expressed inE.coli. The protein consists of the all six soluble domains of the human Wilson protein (residues 1-636 swissprot accession P35670) and contains 3 additional aa (SFT) in the apoform. N-term end of the protein. MW = 67.8 KDa.
||Wilson disease protein (also called ATP7B) is an ATPase that transports copper.This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least two putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
||>90% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight of 66 kDa.
||1mg/ml in sodium phosphate buffer 50mM pH=7.0 , arginine 50mM, glutammic acid 50mM, DTT 2mM, EDTA 1mM, pefabloc® 50uM. The concentration is calculated from the absorbance at 280nm (ε280= 24410 M-1cm-1).
||To avoid precipitation handle the protein in an inert atmosphere, the product can be concentrated to a maximum of 0.3mM.
||metal binding studies, kinetics, WB, SDS-page, blotting, ELISA, antibody production.
||-20ºC. The protein is stable at 25ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.