Recombinant Full Length Human ATP7B Protein, C-Flag-tagged

• Cat.No.: ATP7B-33HFL
• Product Overview: Recombinant Full Length Human ATP7B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein is a monomer, and functions as a copper-transporting ATPase which exports copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease which is characterized by copper accumulation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 157.1 kDa
• AA Sequence: MPEQERQITAREGASRKILSKLSLP TRAWEPAMKKSFAFDNVGYEGGLDG LGPSSQVATSTVRILGMTCQ SCVKSIEDRISNLKGIISMKVSLEQ GSATVKYVPSVVCLQQVCHQIGDMG FEASIAEGKAASWPSRSLPA QEAVVKLRVEGMTCQSCVSSIEGKV RKLQGVVRVKVSLSNQEAVITYQPY LIQPEDLRDHVNDMGFEAAI KSKVAPLSLGPIDIERLQSTNPKRP LSSANQNFNNSETLGHQGSHVVTLQ LRIDGMHCKSCVLNIEENIG QLLGVQSIQVSLENKTAQVKYDPSC TSPVALQRAIEALPPGNFKVSLPDG AEGSGTDHRSSSSHSPGSPP RNQVQGTCSTTLIAIAGMTCASCVH SIEGMISQLEGVQQISVSLAEGTAT VLYNPAVISPEELRAAIEDM GFEASVVSESCSTNPLGNHSAGNSM VQTTDGTPTSLQEVAPHTGRLPANH APDILAKSPQSTRAVAPQKC FLQIKGMTCASCVSNIERNLQKEAG VLSVLVALMAGKAEIKYDPEVIQPL EIAQFIQDLGFEAAVMEDYA GSDGNIELTITGMTCASCVHNIESK LTRTNGITYASVALATSKALVKFDP EIIGPRDIIKIIEEIGFHAS LAQRNPNAHHLDHKMEIKQWKKSFL CSLVFGIPVMALMIYMLIPSNEPHQ SMVLDHNIIPGLSILNLIFF ILCTFVQLLGGWYFYVQAYKSLRHR SANMDVLIVLATSIAYVYSLVILVV AVAEKAERSPVTFFDTPPML FVFIALGRWLEHLAKSKTSEALAKL MSLQATEATVVTLGEDNLIIREEQV PMELVQRGDIVRVVPGGKFP VDGKVLEGNTMADESLITGEAMPVT KKPGSTVIAGSINAHGSVLIKATHV GNDTTLAQIVKLVEEAQMSK APIQQLADRFSGYFVPFIIIMSTLT LVVWIVIGFIDFGVVQKYFPNPNKH ISQTEVIIRFAFQTSITVLC IACPCSLGLATPTAVMVGTGVAAQN GILIKGGKPLEMAHKIKTVMFDKTG TITHGVPRVMRVLLLGDVAT LPLRKVLAVVGTAEASSEHPLGVAV TKYCKEELGTETLGYCTDFQAVPGC GIGCKVSNVEGILAHSERPL SAPASHLNEAGSLPAEKDAAPQTFS VLIGNREWLRRNGLTISSDVSDAMT DHEMKGQTAILVAIDGVLCG MIAIADAVKQEAALAVHTLQSMGVD VVLITGDNRKTARAIATQVGINKVF AEVLPSHKVAKVQELQNKGK KVAMVGDGVNDSPALAQADMGVAIG TGTDVAIEAADVVLIRNDLLDVVAS IHLSKRTVRRIRINLVLALI YNLVGIPIAAGVFMPIGIVLQPWMG SAAMAASSVSVVLSSLQLKCYKKPD LERYEAQAHGHMKPLTASQV SVHIGMDDRWRDSPRATPWDQVSYV SQVSLSSLTSDKPSRHSAAADDDGD KWSLLLNGRDEEQYITRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: ATP7B ATPase copper transporting beta [ Homo sapiens (human) ]
• Official Symbol: ATP7B
• Synonyms: WD; PWD; WC1; WND
• Gene ID: 540
• mRNA Refseq: NM_000053.4
• Protein Refseq: NP_000044.2
• MIM: 606882
• UniProt ID: P35670