Recombinant Human ATP7B Protein, His-tagged

• Cat.No.: ATP7B-467H
• Product Overview: Recombinant Human ATP7B(Thr59~Val426) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 42.9kDa
• Protein length: Thr59~Val426
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 98%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: ATP7B ATPase, Cu++ transporting, beta polypeptide [ Homo sapiens ]
• Official Symbol: ATP7B
• Synonyms: ATP7B; ATPase, Cu++ transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide (Wilson disease) , WND; copper-transporting ATPase 2; Wilson disease; copper pump 2; Wilson disease-associated protein; ATPase, Cu(2+)- transporting, beta polypeptide; WD; PWD; WC1; WND
• Gene ID: 540
• mRNA Refseq: NM_000053
• Protein Refseq: NP_000044
• MIM: 606882
• UniProt ID: P35670