||Recombinant SAP18 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW =19.7 kDa (173 aa).
||SAP18, also known as Sin3A-associated protein, is component of the histone deacetylase complex that plays an important role in the regulation of eukaryotic gene expression. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. It also has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid.
|Amino Acid Sequence:
||MGSSHHHHHH SSGLVPRGSH MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY
||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl,
||> 90% by SDS – PAGE.
||0.5 mg/ml (determined by Bradford assay).
||< 1.0 EU per 1 μg of protein (determined by LAL method ).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.