Recombinant Human Fibroblast Growth Factor 20 FGF20-178H

Recombinant Human Fibroblast Growth Factor 20

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Recombinant Human Fibroblast Growth Factor 20

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Cat. No. : FGF20-178H
Product Overview : This recombinant Human FGF-20 is a 23.2 kDa protein containing 209 amino acid residues and it was expressed inE.Coli.
Description : Fibroblast Growth Factor-20 (FGF-20). The FGF family plays important roles in morphogenesis, cellular differentiation, angiogenesis, tissue remodeling, inflammation and oncogenesis. Fibroblast Growth Factor-20 (FGF-20) is a newly discovered neurotrophic factor belonging to the FGF family. FGF-20 is most similar to FGF-9 and FGF-16.
Source : E.Coli.
Supplied : Sterile filtered and the the protein was lyophilized from 20mM Tris, pH7.6 & 1.5M NaCl (no additives).
Purity : >97% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-20.
Reconstitution : The lyophilized FGF-20 should be reconstituted in 20mM TRIS, pH7.6 or DI water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and stored at 4°Cfor up to 1 week or stored at -20℃ for future use.
Note : If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration greater than 10ug/ml.
Reactivity : The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 10 ng/ml, corresponding to a specific activity of > 1 x 105units/mg.
Amino Acid Sequence : PLAEVGGFLG GLEGLGQQVG SHFLLPPAGE RPPLLGERRS AAERSARGGP GAAQLAHLHG ILRRRQLYCR TGFHLQILPD GSVQGTRQDH SLFGILEFIS VAVGLVSIRG VDSGLYLGMN DKGELYGSEK LTSECIFREQ FEENWYNTYS SNIYKHGDTG RRYFVALNKD GTPRDGARSK RHQKFTHFLP RPVDPERVPE LYKDLLMYT VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK
Storage : The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted human FGF-20 should be stored in working aliquots at -20°C.
Gene Name : FGF20 fibroblast growth factor 20 [ Homo sapiens ]
Synonyms : FGF20; fibroblast growth factor 20; FGF-20
Gene ID : 26281
mRNA Refseq : NM_019851
Protein Refseq : NP_062825
MIM : 605558
UniProt ID : Q9NP95
Chromosome Location : 8p22
Pathway : MAPK signaling pathway; Melanoma; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
Function : growth factor activity

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