| Cat. No. : |
IL17F-114H |
| Description : |
Interleukin-17 (IL-17) is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with one N-linked glycosylation site. IL-17 is also referred to as IL-17A. Other members include IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 binds to the cell surfacer receptor IL-17R. IL-17 is secreted by a subset of T cells called T-helper 17 (Th-17) cells. IL-23 is thought to mediate the production of IL-17 by Th-17 cells. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
RKIPKVGHTFFQKPESCPPVPGGSM KLDIGIINENQRVSMSRNIESRSTS PWNYTVTWDPNRYPSEVVQAQCRNL GCINAQGKEDISMNSVPIQQETLVV RRKHQGCSVSFQLEKVLVTVGCTCV TPVIHHVQ. |
| Molecular Mass : |
Under reducing conditions Apollo IL-17F hcx migrates as a broad band between 17 and 21 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL-17F polypeptide that has a predicted monomeric molecular mass of 14.9 kDa. |
| pI : |
Apollo IL-17F hcx separates into a number of glycoforms with a pI between 6.2 and 9.5 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-17F that has a predicted pI of 9.04. |
| % Carbohydrate : |
Apollo purified IL-17F hcx consists of 20-35% carbohydrate by weight. |
| Glycosylation : |
Apollo IL-17F hcx contains N-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended. |
| Activity : |
The activity of IL-17F is measured by its ability to induce IL-6 production in NHDF cells. The ED50is typically between 0.04 and 0.1 ng/ml. |
