Recombinant Human ACVR2B, Fc tagged

• Cat.No.: ACVR2B-196H
• Product Overview: Recombinant Human ACVR2B extracellular domain N-terminal segment (Met 1-Thr 134) (NP_001097.2), fused with human IgG1 Fc region at the C-terminus, was produced in Human Cell.

Specification

• Source: Human Cells
• Species: Human
• Tag: Fc
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human ACVR2B/Fc chimera is a disulfide-linked homodimer generated after removal of the signal peptide. The reduced monomer comprises 354 amino acids and predicts a molecular mass of 40.0 kDa. As a result of glycosylation, the monomer migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publication:
  Combined detection of the ActRII‐Fc fusion proteins Sotatercept (ActRIIA‐Fc) and Luspatercept (modified ActRIIB‐Fc) in serum by means of immunoaffinity purification, tryptic digestion, and LC‐MS/MS (2018)
  Development of two complementary LC–HRMS methods for analyzing sotatercept in dried blood spots for doping controls (2019)

Gene Information

• Gene Name: ACVR2B activin A receptor, type IIB [ Homo sapiens ]
• Official Symbol: ACVR2B
• Gene ID: 93
• mRNA Refseq: NM_001106
• Protein Refseq: NP_001097
• MIM: 602730
• UniProt ID: Q13705