Active Recombinant Human/Cynomolgus ACVR2B protein, hFc-tagged, Low Endotoxin

• Cat.No.: ACVR2B-196H
• Product Overview: Active Recombinant Human/Cynomolgus ACVR2B protein(Q13705-1(Human) / XP_045242398.1(Cynomolgus))(Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)) is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.

Specification

• Species: Human/Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: 19-137 aa(Human)/40-158 aa(Cynomolgus)
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human/Mouse/Rat Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• Publications:
  • Combined detection of the ActRII‐Fc fusion proteins Sotatercept (ActRIIA‐Fc) and Luspatercept (modified ActRIIB‐Fc) in serum by means of immunoaffinity purification, tryptic digestion, and LC‐MS/MS (2018)
  • Development of two complementary LC–HRMS methods for analyzing sotatercept in dried blood spots for doping controls (2019)

Gene Information

• Gene Name: ACVR2B activin A receptor, type IIB [ Homo sapiens ]
• Official Symbol: ACVR2B
• Synonyms: ACVR2B; activin A receptor, type IIB; activin receptor type-2B; ActR IIB; HTX4; ACTRIIB; ActR-IIB
• Gene ID: 93
• mRNA Refseq: NM_001106
• Protein Refseq: NP_001097
• MIM: 602730
• UniProt ID: Q13705