Active Recombinant Human IGFBP4 protein, His-tagged

• Cat.No.: IGFBP4-759H
• Product Overview: Recombinant Human IGFBP4 protein(NP_001543.2)(Met1-Glu258), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human IGF1 in functional ELISA. 2. Measured by its ability to bind human IGF2 in functional ELISA.3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.04-0.4 μg/mL in the presence of 14 ng/mL human IGFII.
• Molecular Mass: The secreted recombinant human IGFBP4 comprises 248 amino acids with a predicted molecular mass of 27.4 kDa. As a result of glycosylation, it migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Glu258
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IGFBP4 insulin-like growth factor binding protein 4 [ Homo sapiens ]
• Official Symbol: IGFBP4
• Synonyms: IGFBP4; insulin-like growth factor binding protein 4; insulin like growth factor binding protein 4; insulin-like growth factor-binding protein 4; BP 4; HT29 IGFBP; IBP4; IGF binding protein 4; IGFBP 4; IBP-4; IGF-binding protein 4; BP-4; IGFBP-4; HT29-IGFBP
• Gene ID: 3487
• mRNA Refseq: NM_001552
• Protein Refseq: NP_001543
• MIM: 146733
• UniProt ID: P22692