||Recombinant Human IL1F6encoding the human IL1F6 (Met1-Phe158) was expressed inE.coli.It was recombined with a Hexa-his tag at the N-Terminus and purified by Ni-NTA column. MW=17.7 KD.
||IL-1F6 is a secreted cytokine that expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.
||Human IL1F6 (Met1–Phe158)1 MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF 158
||Lyophilized 100μg humanIL1F6 in 50μl of TBS (20mM Tris, 50mM NaCl, pH8.0). Carry free.
||>95% by SDS gel.
||Add 500μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
||Store lyophilized protein at -20℃ or -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at -20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.