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Recombinant Human Transforming Growth Factor, Beta 1, His-tagged

Cat. No.: TGFB1-908H
Product Overview: Recombinant Humantransforming growth factor, beta 1 encoding the human LAP/Prepro-transforming growth factor-beta 1 (Leu30-Arg278) was recombined with his tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=27.4.7KD.
Description: Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis.
Source: E. Coli.
Sequence: Human LAP/Prepro-transforming growth factor-beta 1 (Leu30-Arg278).LSTCKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA GESAEPEPEP EADYYAKEVT RVLMVETHNE IYDKFKQSTH SIYMFFNTSE LREAVPEPVL LSRAELRLLR LKLKVEQHVE LYQKYSNNSW RYLSNRLLAP SDSPEWLSFD VTGVVRQWLS RGGEIEGFRL SAHCSCDSRD NTLQVDINGF TTGRRGDLAT IHGMNRPFLL LMATPLERAQ HLQSSRHRR.
Purity: >95% in SDS gel.
Formulation: Lyophilized 100 μg human LAP/Prepro-transforming growth factor-beta 1in 50 μl of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ). Carry free.
Reconstitution: Add 500μl deionized water to the vial to prepare a working stock solution at 200 μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
Application: Cell biology, ELISA.
Storage: Store lyophilized protein at -20℃ to -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at - 20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for up to a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freeze / thaw cycles.
Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
Synonyms: TGFB1; transforming growth factor, beta 1; CED; LAP; DPD1; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; Camurati-Engelmann disease; Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP
Gene ID: 7040
mRNA Refseq: NM_000660
Protein Refseq: NP_000651
MIM: 190180
UniProt ID: P01137
Chromosome Location: 19q13.1
Pathway: Cell cycle; Chronic myeloid leukemia; Colorectal cancer; Cytokine-cytokine receptor interaction; Hypertrophic cardiomyopathy (HCM); MAPK signaling pathway; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; TGF-beta signaling pathway; Hemostasis; Influenza Infection; Signaling by TGF beta
Function: growth factor activity; protein N-terminus binding; protein heterodimerization activity; protein homodimerization activity; transcription activator activity; type II transforming growth factor beta receptor binding; type II transforming growth factor beta receptor binding

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