||Recombinant Humantransforming growth factor, beta 1 encoding the human LAP/Prepro-transforming growth factor-beta 1 (Leu30-Arg278) was recombined with his tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=27.4.7KD.
||Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis.
||Human LAP/Prepro-transforming growth factor-beta 1 (Leu30-Arg278).LSTCKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA GESAEPEPEP EADYYAKEVT RVLMVETHNE IYDKFKQSTH SIYMFFNTSE LREAVPEPVL LSRAELRLLR LKLKVEQHVE LYQKYSNNSW RYLSNRLLAP SDSPEWLSFD VTGVVRQWLS RGGEIEGFRL SAHCSCDSRD NTLQVDINGF TTGRRGDLAT IHGMNRPFLL LMATPLERAQ HLQSSRHRR.
||>95% in SDS gel.
||Lyophilized 100 μg human LAP/Prepro-transforming growth factor-beta 1in 50 μl of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ). Carry free.
||Add 500μl deionized water to the vial to prepare a working stock solution at 200 μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
||Cell biology, ELISA.
||Store lyophilized protein at -20℃ to -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at - 20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for up to a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freeze / thaw cycles.