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Recombinant Human Leptin Receptor

Cat. No.: LEPR-1249H
Product Overview: Recombinant Human Leptin Binding Domain also called Leptin soluble receptor produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 428-635 of human leptin receptor. The Leptin Binding Domain is purified by proprietary chromatographic techniques.
Description: Leptin receptor (LEPR), also known as OB-R and B219, is a single-transmembrane domain receptor of the cytokine receptor family. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is expressed at high levels in hematopoietic stem cells, lymphohematopoietic cell lines, as well as adult reproductive organs. Several isoforms of LEPR have been identified, and LEPR structurally contains two hemopoietin receptor domains, a fibronectin type III domain and a WSXWS domain within the extracellular region. Interaction of leptin and leptin receptor is crucial for body weight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Solubility: The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino Acid Sequence: The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val.
Biological Activity: Biological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
Stability: Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C. Please prevent freeze-thaw cycles.
Gene Name: LEPR leptin receptor [ Homo sapiens ]
Synonyms: LEPR; leptin receptor; OBR; CD295; OB receptor; DB; Leptin receptor; CD295 antigen; LEP-R; OB-R; HuB219; OTTHUMP00000010694; OTTHUMP00000010693
Gene ID: 3953
mRNA Refseq: NM_001003679
Protein Refseq: NP_001003679
MIM: 601007
UniProt ID: P48357
Chromosome Location: 1p31
Pathway: Adipocytokine signaling pathway; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction
Function: cytokine receptor activity; peptide hormone binding; protein binding; protein-hormone receptor activity

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