||Recombinant, human soluble APO-1/Fas:Fc-IgG (aa.1-154) is produced in human embryo kidney cells. MW= 60 kDa.
||Apo-1 (Fas/CD95) is a 45 kD type I transmembrane protein belonging to the TNF receptor family. A subgroup of this receptor family called ”death receptors” contain 3-4 cysteine-rich repeats in the extracellular portion and a cystoplasmic motif called the ”death domain”, which is responsible for the transduction of the death signal. The death domain associates with FADD/MORT, which in turn connects to aspartate-specific proteases (Caspases) that are implicated as mediators of most types of apoptotic cell death (6-9).
||Human embryo kidney cells.
|Presentation And Handling:
||sAPO-1/Fas is provided as lyophilized powder. Prepare a concentrated stock solution of APO-1/Fas:Fc-IgG (1 mg/ml in PBS) by dissolving the entire preparation (50 µg) in 50 µl sterile H2O. Further dilutions should be made with medium containing 5% fetal calf serum.
||>95% pure as demonstrated by SDS-PAGE.
||Recombinant, human soluble APO-1/Fas:Fc-IgG inhibits the activity of APO-1/Fas ligand of human and mouse.