||Recombinant full-length human PRKCQ was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=110 kDa.
||Protein Kinase C, theta (PKCθ) is important component in the intracellular signaling cascade. Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle. Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes.
||Sf9 insect cells using baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability:
||Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.