Recombinant Human ROCK2, GST-tagged, Active

• Cat.No.: ROCK2-421H
• Product Overview: Recombinant human ROCK2 (5-554) was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=88kDa.

Specification

• Cat. No.: ROCK2-421H
• Description: ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. ROCK2 is an immediate downstream target of the small GTPase RhoA. ROCK2 may play a pivotal role in cardiovascular diseases such as vasospastic angina, ischemic stroke, and heart failure. Inhibition of ROCKs by statins or other selective inhibitors leads to the upregulation and activation of endothelial nitric oxide synthase (eNOS) and reduction of vascular inflammation and atherosclerosis.
• Source: Sf9 insect cells using baculovirus.
• Sequence: 5-554.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: ROCK2 Rho-associated, coiled-coil containing protein kinase 2 [ Homo sapiens ]
• Synonyms: ROCK2; Rho-associated, coiled-coil containing protein kinase 2; KIAA0619; EC 2.7.11.1
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• UniProt ID: O75116
• Chromosome Location: 2p24
• Pathway: Axon guidance; Chemokine signaling pathway; Focal adhesion; Leukocyte transendothelial migration; Pathogenic Escherichia coli infection; Regulation of actin cytoskeleton; TGF-beta signaling pathway; Vascular smooth muscle contraction; Wnt signaling pathway; Signaling by GPCR; Axon guidance
• Function: ATP binding; GTP-Rho binding; identical protein binding; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity; zinc ion binding; transferase activity